J Virol Methods. 2008 Nov 6; : 18996414 (P,S,G,E,B,D) [Cited?]Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses.
[My paper] C F Gambley, A D W Geering, J E Thomas
Department of Primary Industries and Fisheries, Horticulture and Forestry Science, 80 Meiers Road, Indooroopilly, Queensland 4068, Australia; School of Integrative Biology, The University of Queensland, St. Lucia, Queensland 4072, Australia.
A semi-automated, immunomagnetic capture-reverse-transcription PCR (IMC-RT-PCR) assay for the detection of three pineapple-infecting ampeloviruses, Pineapple mealybug wilt-associated virus-1, -2 and -3, is described. The assay was equivalent in sensitivity but more rapid than conventional immunocapture RT-PCR. The assay can be used either as a one- or two-step RT-PCR and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf tissue. This IMC-RT-PCR assay could be used for high throughput screening of pineapple planting propagules and could easily be modified for the detection of other RNA viruses in a range of plant species, provided suitable antibodies are available.
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